Journal: International Journal of Molecular Sciences

Article Title: Intraarticularly-Injected Mesenchymal Stem Cells Stimulate Anti-Inflammatory Molecules and Inhibit Pain Related Protein and Chondrolytic Enzymes in a Monoiodoacetate-Induced Rat Arthritis Model

doi: 10.3390/ijms19010203

Figure Lengend Snippet: ADAMTS5 expression in cartilage tissue. ( a ) control group, ( b ) Saline group, ( c ) MIA+/MSC− group, ( d ) MIA+/MSC+ group. Control group and Saline group: Almost no ADAMTS5 is found in cartilage cells. MIA+/MSC− group: ADAMTS5 is extensively expressed from the hypertrophied cartilage layer to surface layer of cartilage cells. MIA+/MSC+ group: ADAMTS5 expression in cartilage cells is greater than in control and Saline group, but as compared with MIA+/MSC− group, ADAMTS5 expression is diffuse and clearly. A significant increase in ADAMTS5 expression is found in the MIA+/MSC− group. In the MIA+/MSC+ group as compared with A, B, it is increased, while as compared with the MIA+/MSC− group it is significantly decreased. * p < 0.001 compared to the untreated control. # p < 0.001 compared to the (MIA+/MSC−) group.

Article Snippet: For antigen retrieval, the sections were dipped into the protease K at 37 °C for 30 min. After washing in PBS, endogenous peroxidase activity was inhibited with normal goat or rabbit serum (Nichirei Co., Tokyo, Japan) for 30 min. Tissue sections were incubated overnight at 4 °C with affinity-purified anti-ADAMTs5 or anti-TSG6 rabbit polyclonal antibodies (Funakoshi, Tokyo, Japan), (abcam204049, Tokyo, Japan) at a concentration of 10 μg/mL each.

Techniques: Expressing